Journal: ACS chemical neuroscience

Article Title: Passage Variation of PC12 Cells Results in Inconsistent Susceptibility to Externally Induced Apoptosis

doi: 10.1021/acschemneuro.6b00208

Figure Lengend Snippet: Optimization and validation of etoposide-induced cell death in PC12 cells (passage 9–13). 50 × 103 cells/well. (a) Approximately 50% cell death was observed after 48 h. Four replicates per experiment. Values are represented as mean ± SD. Multiple t test, Holm-Sidak method, alpha = 5.000%, ****p < 0.0001. (b) Fluorescence measured after 48 h. Flupirtine at 3 μM concentration rescued the cells from apoptosis. Experiment repeated thrice. Values are represented as mean ± SD. Unpaired two-tailed t test, 95% CI, ****p < 0.0001 in comparison to vehicle. ####p < 0.0001 in comparison to etoposide treatment.

Article Snippet: 5 Etoposide (Chem-Impex International, 28435) was stored as a working stock solution of 150 μ g/mL at −20 °C for up to 3 months.

Techniques: Fluorescence, Concentration Assay, Two Tailed Test, Comparison

Journal: ACS chemical neuroscience

Article Title: Passage Variation of PC12 Cells Results in Inconsistent Susceptibility to Externally Induced Apoptosis

doi: 10.1021/acschemneuro.6b00208

Figure Lengend Snippet: NGF differentiated PC12 cells show a statistically significant difference in sensitivity to apoptosis induced by etoposide between early and late passages at 72 h post insult. (a) Passage 10. Three replicates per experiment. Values are represented as mean ± SD. One-way ANOVA, Dunnett test, 95% CI. ****p < 0.0001. (b) Passage 17. Three replicates per experiment. Values are represented as mean ± SD. One-way ANOVA, Dunnett test, 95% CI. ****p < 0.0001. (c) Comparison of 72 h etoposide treatment in passage 10 and 17 PC12 cells differentiated using NGF. Unpaired two-tailed t test, 95% CI, ****p < 0.0001.

Article Snippet: 5 Etoposide (Chem-Impex International, 28435) was stored as a working stock solution of 150 μ g/mL at −20 °C for up to 3 months.

Techniques: Comparison, Two Tailed Test

Journal: Nature Communications

Article Title: Efficient intracellular delivery of proteins by a multifunctional chimaeric peptide in vitro and in vivo

doi: 10.1038/s41467-021-25448-z

Figure Lengend Snippet: a , b eTAT-Ppm1b treatment of APAP-induced acute liver damage. Overnight-fasted female BALB/c mice were challenged intraperitoneally with APAP at a dose of 500 mg per kg body weight and then treated intravenously with Ppm1b-related recombinant proteins twice, 2 and 6 h after APAP administration ( a ). Twelve hours post-APAP challenge, serum was collected, and ALT and AST levels were determined ( b ). Mice treated with PBS were used as negative controls, and those challenged with DMSO solution and treated with PBS served as solvent controls. The mock mice received neither APAP nor Ppm1b-related proteins. All the values are expressed as the means ± s.e.m., n = 7 for each group; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and NS no significant difference; two-tailed unpaired student’s t test. c, d eTAT-Ppm1b therapy for attenuating APAP-induced death. Overnight-fasted female BALB/c mice were challenged intraperitoneally with APAP at a dose of 800 mg per kg body weight and then treated intravenously with Ppm1b-related recombinant proteins twice, 2 and 6 h after APAP administration ( c ). Then, the survival of the mice was monitored every hour for 72 h with the results presented in a Kaplan–Meier plot, and a log-rank (Mantel-Cox) test was performed; * P < 0.05, ** P < 0.01 ( d ). Mice treated with PBS were used as negative controls; n = 16 mice for each group pooled from two independent experiments. For data, statistics, and exact P values, see Source Data File.

Article Snippet: Acetaminophen (APAP) and N-acetylcysteine amide (NAC) were obtained from MedChemExpress (MCE).

Techniques: Recombinant, Solvent, Two Tailed Test